Effects of IL-4 and IL-13 on Colon Cancer Cell Adhesion
Author Information
Author(s): Kanai T, Watanabe M, Hayashi A, Nakazawa A, Yajima T, Okazawa A, Yamazaki M, Ishii H, Hibi T
Primary Institution: Keio Cancer Center and Department of Internal Medicine, School of Medicine, Keio University
Hypothesis
To assess the role of interleukin-4 (IL-4) and interleukin-13 (IL-13) in colon cancer cell–cell adhesion.
Conclusion
IL-4 and IL-13 inhibit colon cancer cell–cell adhesion by down-regulating E-cadherin and CEA molecules.
Supporting Evidence
- IL-4 receptor was expressed on the cell surface of colo205.
- Recombinant IL-4 inhibited colo205 cell–cell adhesion in a dose-dependent fashion.
- Monoclonal antibodies against E-cadherin and CEA inhibited colo205 cell–cell adhesion.
- IL-4 significantly inhibited the expression of E-cadherin and CEA.
- IL-13 also inhibited colo205 cell–cell adhesion.
- Colon TILs expressed IL-4 and IL-13 mRNA and protein.
Takeaway
This study found that two proteins, IL-4 and IL-13, can stop colon cancer cells from sticking together, which might help us understand how cancer spreads.
Methodology
Investigated the effect of IL-4 and IL-13 on human colon cancer cell line colo205 using flow cytometric analysis and reverse transcription-polymerase chain reaction.
Digital Object Identifier (DOI)
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