Effects of Donor Age and Cell Passage on Stem Cell Differentiation
Author Information
Author(s): Kretlow James D, Jin Yu-Qing, Liu Wei, Zhang Wen Jie, Hong Tan-Hui, Zhou Guangdong, Baggett L Scott, Mikos Antonios G, Cao Yilin
Primary Institution: Rice University
Hypothesis
How do donor age and cell passage affect the differentiation potential of murine bone marrow-derived stem cells?
Conclusion
Both increasing age and the number of passages have lineage dependent effects on BMSC differentiation potential.
Supporting Evidence
- Cells from younger donors adhered better and proliferated more than those from older animals.
- Chondrogenic and osteogenic potential decreased with age.
- Adipogenic differentiation decreased only in cells from the oldest donors.
- Significant decreases in differentiation potentials due to passage were observed.
Takeaway
As mice get older and as stem cells are passed through more generations in the lab, their ability to turn into different types of cells decreases.
Methodology
The study used a factorial design to investigate the differentiation of murine BMSCs from donors of different ages and passages.
Limitations
The study primarily focused on murine models, which may not fully represent human stem cell behavior.
Participant Demographics
Murine bone marrow-derived stem cells from postnatal, adult, and aged mice.
Statistical Information
P-Value
p<0.0001
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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