Fluoride Causes Stress in Cells and Affects Protein Production
Author Information
Author(s): R. Sharma, M. Tsuchiya, J.D. Bartlett
Primary Institution: Forsyth Institute
Hypothesis
Does low-dose fluoride cause endoplasmic reticulum stress and affect protein secretion in ameloblasts?
Conclusion
Fluoride induces endoplasmic reticulum stress in ameloblasts, impairing their function and potentially leading to dental fluorosis.
Supporting Evidence
- Fluoride decreases the secretion of a reporter protein in a dose-dependent manner.
- Increased intracellular accumulation of the reporter protein was observed after fluoride exposure.
- Fluoride treatment activated the unfolded protein response in ameloblasts.
Takeaway
Fluoride can make it hard for certain cells in your teeth to do their job, which might lead to problems like discolored teeth.
Methodology
The study used a cell line transfected with a reporter gene to measure protein secretion and assessed the effects of fluoride on protein synthesis and stress response.
Limitations
The study primarily used in vitro models and may not fully represent in vivo conditions.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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