Treatment of IBD-E. coli in a Mouse Model
Author Information
Author(s): Petersen Andreas Munk, Schjørring Susanne, Gerstrøm Sarah Choi, Krogfelt Karen Angeliki
Primary Institution: Statens Serum Institute, Copenhagen, Denmark
Hypothesis
Can ciprofloxacin and/or E. coli Nissle eradicate IBD associated E. coli in the streptomycin-treated mouse intestine?
Conclusion
Ciprofloxacin effectively eradicated IBD associated E. coli, but E. coli Nissle alone did not, and its introduction after ciprofloxacin treatment allowed some strains to re-colonize.
Supporting Evidence
- Ciprofloxacin treatment for three days effectively eradicated IBD associated E. coli in mice.
- E. coli Nissle did not eradicate IBD associated E. coli but resulted in co-colonization.
- One IBD associated E. coli strain reappeared after treatment with E. coli Nissle.
Takeaway
This study tested if a medicine and a probiotic could get rid of bad bacteria in mice with gut problems. The medicine worked, but the probiotic didn't help on its own.
Methodology
Mice were precolonized with E. coli strains and treated with ciprofloxacin and/or E. coli Nissle, with colonization levels monitored through fecal cultures.
Limitations
The study was conducted in a mouse model, which may not fully replicate human IBD conditions.
Participant Demographics
Mice used were six-to-eight-week-old, outbreed albino female CFW1 mice.
Digital Object Identifier (DOI)
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