How Tumor-Associated Macrophages Help Colorectal Cancer Grow
Author Information
Author(s): Liu Jinlin, Zhang Ning, Li Qun, Zhang Weiwei, Ke Fang, Leng Qibin, Wang Hong, Chen Jinfei, Wang Honglin
Primary Institution: Shanghai Jiao Tong University School of Medicine
Hypothesis
Tumor-associated macrophages (TAMs) contribute to the recruitment of regulatory T cells (Treg-cells) to colorectal cancer (CRC) and promote its development through CCL20 production.
Conclusion
TAMs recruit CCR6+ Treg-cells to tumor mass and promote its development via enhancing the production of CCL20 in a CRC mouse model.
Supporting Evidence
- 85% of mice developed visible colorectal cancer after treatment.
- Treg-cells were significantly increased in tumor-infiltrating lymphocytes compared to normal mucosa.
- Injection of recombinant CCL20 promoted tumor growth and Treg-cell recruitment.
- Selective depletion of macrophages reduced CCL20 levels and inhibited tumor growth.
Takeaway
In mice with colorectal cancer, certain immune cells called macrophages help attract other immune cells that can actually help the cancer grow.
Methodology
Colorectal cancer was induced in mice using N-methyl-N-nitrosourea and H. pylori, and the role of macrophages in Treg-cell recruitment was assessed through various experimental techniques including co-culture and flow cytometry.
Limitations
The study was conducted in a mouse model, which may not fully replicate human colorectal cancer.
Participant Demographics
C57BL/6J mice, 6-week-old females.
Statistical Information
P-Value
p<0.0001
Statistical Significance
p<0.0001
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website