Alternative Mating Type Configurations (a/α versus a/a or α/α) of Candida albicans Result in Alternative Biofilms Regulated by Different Pathways
2011
Different Biofilms of Candida albicans
publication
Evidence: moderate
Author Information
Author(s): Song Yi, Nidhi Sahni, Karla Daniels, David Soll
Hypothesis
Do the two types of biofilms play different roles in the life cycle of C. albicans, and are their biofilms regulated by different signaling pathways?
Conclusion
Heterozygous biofilms are more protective and less permeable than homozygous biofilms, which are more permeable and facilitate mating.
Supporting Evidence
- Heterozygous biofilms were about 28% thicker on average than their homozygous counterparts.
- Heterozygous biofilms were impermeable to white blood cells, while homozygous biofilms were not.
- Heterozygous biofilms had nine times more dead cells after antifungal treatment compared to homozygous biofilms.
- The two biofilms are regulated by distinct pathways.
Takeaway
Candida albicans can make two types of biofilms that act differently; one type protects the cells inside, while the other helps them mate.
Methodology
The authors allowed heterozygous and homozygous biofilms to grow for 48 hours and examined them under a microscope, measuring parameters such as biofilm adhesion, mass, and thickness.
Digital Object Identifier (DOI)
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