Mapping CREM and CREB Binding Sites in Male Germ Cells
Author Information
Author(s): Martianov Igor, Choukrallah Mohamed-Amin, Krebs Arnaud, Ye Tao, Legras Stephanie, Rijkers Erikjan, Van Ijcken Wilfred, Jost Bernard, Sassone-Corsi Paolo, Davidson Irwin
Primary Institution: Institut de Génétique et de Biologie Moléculaire et Cellulaire
Hypothesis
How do CREM and CREB binding sites differ in male germ cells?
Conclusion
CREM and CREB occupy a large number of promoters in a highly cell-specific manner.
Supporting Evidence
- More than 9000 genomic loci were identified as being occupied by CREM and CREB.
- CREM occupied over 6700 target loci in round spermatids.
- The study provides the first comprehensive analysis of CREB/CREM regulatory potential in vivo.
Takeaway
This study looked at how two proteins, CREM and CREB, attach to DNA in male germ cells to help control gene activity.
Methodology
Chromatin immunoprecipitation coupled to sequencing (ChIP-seq) was used to map binding sites.
Limitations
The study may not account for all potential binding sites or regulatory mechanisms.
Participant Demographics
Adult male mice were used for the study.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website