Production and Characterization of Laccase from Trametes trogii in Yeast
Author Information
Author(s): Colao Maria Chiara, Lupino Stefania, Garzillo Anna Maria, Buonocore Vincenzo, Ruzzi Maurizio
Primary Institution: Department of Agrobiology and Agrochemistry, Tuscia University
Hypothesis
Can the lcc1 gene from Trametes trogii be successfully expressed in Pichia pastoris to produce a functional laccase enzyme?
Conclusion
The lcc1 gene from T. trogii was successfully expressed in P. pastoris, resulting in a recombinant laccase with potential industrial applications.
Supporting Evidence
- The recombinant laccase was produced at a yield of 17 mg/l.
- The enzyme showed high activity in the presence of organic solvents.
- Decolourization of synthetic dyes was significantly enhanced with redox mediators.
Takeaway
Scientists took a gene from a fungus and put it into yeast to make an enzyme that can help break down dyes and other pollutants.
Methodology
The lcc1 cDNA was expressed in Pichia pastoris using the alcohol oxidase promoter, and the recombinant enzyme was purified and characterized.
Limitations
The study did not explore the long-term stability of the enzyme in various industrial conditions.
Digital Object Identifier (DOI)
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