Isolation of the Bacteriophage DinoHI from Dichelobacter nodosus
Author Information
Author(s): Cheetham Brian F, Parker Dane, Bloomfield Garry A, Shaw Bruce E, Sutherland Megan, Hyman Jessica A, Druitt Jenifer, Kennan Ruth M, Rood Julian I, Katz Margaret E
Primary Institution: University of New England
Hypothesis
The study investigates the role of the bacteriophage DinoHI in the genetic interactions of Dichelobacter nodosus.
Conclusion
The bacteriophage DinoHI may play a role in the transfer of the virulence-related locus (vrl) by transduction.
Supporting Evidence
- The DinoHI genome contains a copy of regA, suggesting a regulatory role.
- DinoHI is the first bacteriophage identified in D. nodosus.
- Ultraviolet light treatment successfully induced the bacteriophage from D. nodosus strains.
- Electron microscopy revealed the structure of the DinoHI phage particles.
- Southern blot analysis indicated the presence of integrated copies of DinoHI in several D. nodosus strains.
Takeaway
Scientists found a new virus called DinoHI in a bacteria that causes footrot in sheep, and it might help spread a gene that makes the bacteria more harmful.
Methodology
The study involved isolating the bacteriophage from D. nodosus strains using ultraviolet light and mitomycin C, followed by genomic analysis.
Digital Object Identifier (DOI)
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