Bacterial Lipopolysaccharide Potentiates Type II Collagen-Induced Arthritis in Mice
Author Information
Author(s): Robert G. CaccesecA, John L. Zirnmerman, Richard P. Carlson
Primary Institution: Wyeth-Ayerst Research, Princeton, NJ, USA
Hypothesis
Does bacterial lipopolysaccharide (LPS) enhance the severity and incidence of collagen-induced arthritis in DBA/1LacJ mice?
Conclusion
LPS significantly accelerates the onset and increases the severity of collagen-induced arthritis in female DBA/1LacJ mice.
Supporting Evidence
- Female mice had an earlier onset of disease and higher disease incidence than male mice.
- LPS treatment accelerated the onset of disease from day 35 to day 21.
- Serum amyloid P levels were significantly higher in LPS treated mice compared to non-treated mice.
- There was a significant correlation between SAP levels and disease expression in LPS treated mice.
Takeaway
This study found that a substance called LPS makes mice get arthritis faster and worse when they are given a type of collagen.
Methodology
DBA/1LacJ mice were immunized with type II collagen and treated with LPS or IL-1 to assess the effects on arthritis onset and severity.
Potential Biases
Potential bias due to the controlled housing conditions and the specific strain of mice used.
Limitations
The study was conducted in a controlled environment, which may not fully represent natural conditions.
Participant Demographics
DBA/1LacJ mice, 5-6 weeks old, both male and female.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Want to read the original?
Access the complete publication on the publisher's website