Microvascular Engineering in Perfusion Culture
Author Information
Author(s): Frerich Bernhard, Zückmantel Kerstin, Hemprich Alexander
Primary Institution: University of Leipzig
Hypothesis
Can pulsatile perfusion enhance the development of vascular networks in engineered tissue?
Conclusion
Pulsatile perfusion supports the development of vascular networks with a physiological appearance.
Supporting Evidence
- Perfused specimens showed a round or oval lumen lined by a single layer of endothelial cells.
- Confocal laser scanning microscopy showed more extended network formation in perfused specimens.
- Perfused specimens exhibited significantly more branches emerging from the central lumen.
Takeaway
This study shows that using a special flow method helps create better blood vessel structures in lab-made tissues.
Methodology
An in vitro model was developed using adipose tissue stromal cells and human umbilical vein endothelial cells in a gelatine scaffold, comparing pulsatile perfusion culture to rotation culture over 16 days.
Potential Biases
Potential variability in histological appearance and mural cell differentiation.
Limitations
Variability in results and difficulty in quantifying pressure and shear forces applied.
Participant Demographics
Adipose tissue collected from routine operations with informed consent from patients.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website