Initiation of human colon cancer cell proliferation by trypsin acting at protease-activated receptor-2
2001
How Trypsin Helps Colon Cancer Cells Grow
Sample size: 10
publication
Evidence: moderate
Author Information
Author(s): Darmoul D, Marie J-C, Devaud H, Gratio V, Laburthe M
Primary Institution: Institut National de la Santé et de la Recherche Médicale, INSERM U410, Faculté de Médecine Xavier Bichât, Paris, 75018, France
Hypothesis
Does trypsin promote the proliferation of human colon cancer cells through protease-activated receptor-2 (PAR-2)?
Conclusion
Trypsin can promote the growth of human colon cancer cells by acting on PAR-2.
Supporting Evidence
- PAR-2 mRNA was detected in 6 out of 10 tested colon cancer cell lines.
- Trypsin and the peptide agonist SLIGKV were shown to induce calcium mobilization.
- Cells treated with trypsin or SLIGKV showed a threefold increase in cell number.
Takeaway
This study found that a substance called trypsin can help colon cancer cells grow bigger and faster.
Methodology
The study tested 10 human colon cancer cell lines for PAR-2 expression and assessed the effects of trypsin and a peptide agonist on cell proliferation.
Digital Object Identifier (DOI)
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