Comparing Old and New SELDI-TOF MS for Serum Protein Profiling
Author Information
Author(s): Marie-Christine Gast, Judith Engwegen, Jan HM Schellens, Jos H Beijnen
Primary Institution: The Netherlands Cancer Institute
Hypothesis
The new PCS 4000 SELDI-TOF MS instrument will outperform the old PBS-IIc in serum protein profiling.
Conclusion
The PCS 4000 instrument showed superior performance in peak detection after serum fractionation, but did not outperform the PBS-IIc in crude serum analysis.
Supporting Evidence
- The PCS 4000 detected more peaks in serum fractions compared to the PBS-IIc.
- Both instruments were tested on two different cancer patient cohorts.
- The study found no significant difference in performance for crude serum analysis.
Takeaway
Scientists tested two machines that look at proteins in blood to see which one is better. The new machine is better at finding certain proteins when the blood is treated in a special way.
Methodology
Two sample sets were profiled using both SELDI-TOF MS instruments, and the performance was evaluated based on the number of peaks detected and their reproducibility.
Limitations
The study could not confirm the improved performance of the PCS 4000 over the PBS-IIc in crude serum analysis.
Participant Demographics
The study included 45 colorectal cancer patients and 43 matched controls, as well as 45 breast cancer patients and 46 matched normal women.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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