CESA: A Tool for Finding Useful Antibodies in Other Species
Author Information
Author(s): Hu Yanhui, Gao Chenxi, Mckenna William, Xia Baolong, Ariss Majd, Mohr Stephanie, Perrimon Norbert
Primary Institution: Department of Genetics, Blavatnik Institute, Harvard Medical School
Hypothesis
Can existing phospho-specific antibodies developed for mammals be useful for detecting phosphorylated proteins in model organisms?
Conclusion
The CESA tool predicts that many phospho-specific antibodies can be effectively used in Drosophila and potentially other model organisms.
Supporting Evidence
- CESA predicts that more than 232 sites on 116 Drosophila proteins can potentially be targeted by antibodies developed for mammals.
- The tool was able to map 998 of 1,170 genes from human, mouse, and rat to 659 Drosophila genes.
- Gene set enrichment analysis identified over-representation of kinases among the targeted Drosophila genes.
Takeaway
Scientists created a tool called CESA to find antibodies that can work in fruit flies, even though they were originally made for humans and other mammals.
Methodology
The CESA tool was developed to analyze conservation of phospho-specific antibody target sites across species using protein alignment and mapping techniques.
Potential Biases
The analysis primarily focused on antibodies from one commercial source, which may introduce bias in the findings.
Limitations
The tool's predictions are based on existing data, which may not cover all potential cross-reactive antibodies.
Participant Demographics
The study primarily involved Drosophila melanogaster as the model organism.
Statistical Information
P-Value
2.80E-24
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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