Interactions Between Gcn5 and Hda1 in Yeast
Author Information
Author(s): Islam Azharul, Turner Emma L, Menzel Johannes, Malo Mackenzie E, Harkness Troy AA
Primary Institution: University of Saskatchewan
Hypothesis
The study investigates the antagonistic interactions between the histone acetyltransferase Gcn5 and the histone deacetylase Hda1 in the context of the Anaphase Promoting Complex (APC) in yeast.
Conclusion
The study concludes that Gcn5 and Hda1 compete for binding to promoters, influencing gene expression and APC activity.
Supporting Evidence
- Deletion of GCN5 or HDA1 exacerbated the temperature sensitive mutant phenotype of the APC apc5CA allele.
- Hda1 and Gcn5 compete for recruitment to promoters, affecting gene expression.
- Complex formation between Hda1 and Tup1 can occlude Gcn5 recruitment to promoters.
- In apc5CA hda1Δ cells, transcript levels of certain genes were elevated, indicating a regulatory role of Gcn5.
Takeaway
This study shows that two proteins, Gcn5 and Hda1, work against each other to control how genes are turned on or off in yeast.
Methodology
The study utilized various techniques including Northerns, Westerns, reverse transcriptase PCR, chromatin immunoprecipitation, and mutant phenotype suppression analysis.
Digital Object Identifier (DOI)
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