17β-Estradiol Enhances Breast Cancer Cell Motility and Invasion via Extra-Nuclear Activation of Actin-Binding Protein Ezrin
2011
Estrogen Enhances Breast Cancer Cell Movement and Invasion
publication
10 minutes
Evidence: high
Author Information
Author(s): Zheng Shuhui, Huang Jinghe, Zhou Kewen, Zhang Chengxi, Xiang Qiuling, Tan Zhi, Wang Tinghuai, Fu Xiaodong
Primary Institution: Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China
Hypothesis
The study investigates the effects of 17β-estradiol on ezrin activation and its role in breast cancer cell motility and invasion.
Conclusion
17β-estradiol promotes breast cancer cell movement and invasion by activating ezrin.
Supporting Evidence
- E2 rapidly enhances ezrin phosphorylation at Thr567 in a time- and concentration-dependent manner.
- E2 enhances horizontal cell migration and invasion of T47-D breast cancer cells in three-dimensional matrices.
- The signaling cascade involves estrogen receptor interaction with c-Src, activating the PI3K/Akt pathway.
- Silencing ezrin with siRNAs reverses the effects of E2 on cell migration and invasion.
Takeaway
Estrogen helps breast cancer cells move and spread by turning on a protein called ezrin.
Methodology
The study used T47-D breast cancer cells to examine the effects of 17β-estradiol on ezrin phosphorylation and cell migration/invasion.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website