Integrated Metagenomic and Metabolomic Analysis of In Vitro Murine Gut Microbial Cultures upon Bisphenol S Exposure
2024

Impact of Bisphenol S on Gut Microbiota in Mice

Sample size: 5 publication 10 minutes Evidence: moderate

Author Information

Author(s): A. Cox, Nowshad Farrhin, Callaway Evelyn, Jayaraman Arul

Primary Institution: Texas A&M University

Hypothesis

What is the effect of Bisphenol S on the gut microbiota of C57BL/6 mice?

Conclusion

The study found that Bisphenol S exposure did not significantly alter the gut microbiota composition or metabolome in mice.

Supporting Evidence

  • BPS exposure did not produce a distinct microbial profile compared to controls.
  • Several metabolites, including saturated fatty acids, were enriched in BPS-exposed cultures.
  • Lactobacillus species were associated with BPS exposure in a discriminant model.

Takeaway

This study looked at how a chemical called Bisphenol S affects tiny living things in the stomach of mice, and it found that it didn't really change much.

Methodology

The study used shotgun metagenomic sequencing and untargeted LC-MS/MS metabolomic analysis on murine fecal cultures exposed to Bisphenol S.

Potential Biases

Potential bias due to the use of a supraphysiologic dose and the in vitro model not fully representing in vivo conditions.

Limitations

Inter-animal variation was significant, and the lack of an acclimation phase for the microbiota may have affected results.

Participant Demographics

Female C57BL/6 mice were used in the study.

Statistical Information

P-Value

0.605 for metabolomics, 0.989 for metagenomics

Statistical Significance

p=0.605 for metabolomics, p=0.989 for metagenomics

Digital Object Identifier (DOI)

10.3390/metabo14120713

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