TFIP11 Interacts with mDEAH9, an RNA Helicase Involved in Spliceosome Disassembly
2008
TFIP11 and mDEAH9 Interact in Spliceosome Disassembly
publication
Evidence: moderate
Author Information
Author(s): Wen Xin, Tannukit Sissada, Michael L. Paine
Primary Institution: University of Southern California School of Dentistry
Hypothesis
TFIP11 recruits mDEAH9 to facilitate spliceosome disassembly.
Conclusion
TFIP11 and mDEAH9 likely work together in late-stage RNA splicing events.
Supporting Evidence
- TFIP11 and mDEAH9 colocalize in distinct nuclear speckles.
- Mutations in the G-patch domain of TFIP11 do not affect its nuclear localization.
- TFIP11 interacts with mDEAH9 in vitro and in vivo.
Takeaway
This study shows that two proteins, TFIP11 and mDEAH9, help cells cut and process RNA, similar to how a pair of scissors works.
Methodology
The study used plasmid constructs, transfection, immunoprecipitation, and Western blot analysis to investigate protein interactions.
Digital Object Identifier (DOI)
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