Transcriptional analysis of the bglP gene from Streptococcus mutans
2006
Study of the bglP Gene in Streptococcus mutans
publication
Evidence: moderate
Author Information
Author(s): Cote Christopher K, Honeyman Allen L
Primary Institution: University of South Florida, College of Medicine
Hypothesis
Antitermination is the regulatory mechanism responsible for the control of the bglP gene expression.
Conclusion
The study supports that the bglP gene is regulated by antitermination mediated by the LicT protein.
Supporting Evidence
- The LicT protein is essential for optimal transcriptional activity of the bglP promoter region.
- A short terminated mRNA transcript was identified in the absence of the inducer, esculin.
- The bglP gene produces a 2.1 kb transcript in the presence of esculin.
Takeaway
This study found that a protein called LicT helps control a gene in bacteria that breaks down certain sugars, making it work better when those sugars are present.
Methodology
Transcriptional lacZ gene fusions and beta-galactosidase assays were used to analyze promoter activity.
Digital Object Identifier (DOI)
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