A protocol to assess cell cycle and apoptosis in human and mouse pluripotent cells
2011
Standard Protocol for Assessing Cell Cycle and Apoptosis in Pluripotent Cells
publication
Evidence: high
Author Information
Author(s): Edel Michael J, Menchon Cristina, Vaquero Jose Miguel Andres, Izpisua Belmonte Juan Carlos
Primary Institution: Center of Regenerative Medicine in Barcelona
Hypothesis
A standardized method for measuring cell cycle and apoptosis in embryonic and induced pluripotent stem cells is needed.
Conclusion
The study presents a detailed protocol that standardizes the assessment of cell cycle and apoptosis in pluripotent stem cells.
Supporting Evidence
- The protocol allows for concurrent assessment of cell cycle and apoptosis from the same sample.
- Using EdU and DAPI staining provides reliable results for cell cycle analysis.
- The method is applicable to both human and mouse pluripotent stem cells.
Takeaway
This study provides a simple recipe for scientists to check how fast stem cells are growing and if they are healthy.
Methodology
The protocol involves using flow cytometry to measure cell cycle profiles and apoptosis in pluripotent stem cells using specific staining techniques.
Digital Object Identifier (DOI)
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