Expression of Cell-Penetrating Peptide L2 in Yeast
Author Information
Author(s): Li Xuan, Yang Na, Fang Yuxin, Mao Ruoyu, Hao Ya, Teng Da, Dong Na, Shan Anshan, Wang Jianhua
Primary Institution: Chinese Academy of Agricultural Sciences
Hypothesis
Can the cell-penetrating peptide L2 be effectively expressed in Pichia pastoris using SUMO as a fusion partner?
Conclusion
The study found that SUMO is a suitable fusion tag for the high-yield expression of the cell-penetrating peptide L2.
Supporting Evidence
- SUMO-L2 expression reached 629 mg/L in a 5 L fermenter.
- Recombinant L2 showed antibacterial activity with MICs of 4–8 μg/mL against S. agalactiae.
- The study compared the expression levels of SUMO-L2 and 2FLAG-L2, finding SUMO-L2 to be superior.
Takeaway
Scientists figured out how to make a special protein that can get inside cells and kill bad bacteria using yeast, which is cheaper and easier than making it in a lab.
Methodology
The study used heterologous expression in Pichia pastoris with SUMO and a short acidic fusion tag to produce the peptide.
Limitations
The expression of some AMPs can be toxic to the host and may not be suitable for direct expression.
Digital Object Identifier (DOI)
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