Immunoassay for Detecting Benzo[a]pyrene in Grilled Pork
Author Information
Author(s): Jeeno Peerapong, Yadoung Sumed, Thongkham Marninphan, Yana Pichamon, Jaitham Udomsap, Ounjaijean Sakaewan, Xu Zhen-Lin, Sringarm Korawan, Hongsibsong Surat
Primary Institution: Research Institute for Health Sciences, Chiang Mai University
Hypothesis
The study aims to create an immunoassay for the detection of benzo[a]pyrene utilizing immunoglobulin Y antibodies.
Conclusion
The developed immunoassay effectively detects benzo[a]pyrene in grilled pork samples, providing a cost-effective method to protect human health and the environment.
Supporting Evidence
- The immunoassay demonstrated high sensitivity for detecting benzo[a]pyrene in grilled pork samples.
- The recovery rates for spiked samples ranged from 74.99% to 143.11%.
- The IC10 value for benzo[a]pyrene in grilled pork was established at 1.24 µg/kg.
- The developed immunoassay represents a significant advancement in environmental analytical chemistry.
Takeaway
This study created a new test to find a harmful chemical in grilled pork using special antibodies from chicken eggs.
Methodology
An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) was developed using IgY antibodies extracted from egg yolk.
Limitations
The immunoassay showed significant cross-reactivity with benzo[b]fluoranthene, making it challenging to distinguish between these compounds in complex mixtures.
Digital Object Identifier (DOI)
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