The HECT ubiquitin‐protein ligases UPL1 and UPL2 are involved in degradation of Arabidopsis thaliana ACC synthase 7
2025
HECT Ubiquitin-Protein Ligases UPL1 and UPL2 in Arabidopsis
publication
10 minutes
Evidence: high
Author Information
Author(s): Marczak Małgorzata, Cieśla Agata, Janicki Maciej, Mehdi Syed Muhammad Muntazir, Kubiak Piotr, Ludwików Agnieszka
Primary Institution: Adam Mickiewicz University in Poznań
Hypothesis
UPL1 and UPL2 regulate the stability of ACC synthase 7 in Arabidopsis thaliana.
Conclusion
UPL1 and UPL2 E3 ligases are crucial for regulating ACS7 stability and ethylene production in response to stress.
Supporting Evidence
- UPL1 and UPL2 E3 ligases directly control ACS7 turnover.
- Increased ethylene levels were observed in UPL1- and UPL2-knockout plants.
- Cell-free degradation assays showed that UPL1 and UPL2 regulate ACS7 stability.
Takeaway
This study shows that two proteins help control how a plant makes a gas called ethylene, which is important for its growth and response to stress.
Methodology
Mass spectrometry was used to identify components of the ubiquitin system, and cell-free degradation assays were performed to assess the stability of ACS7.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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