Opposite effects of interleukin-4 and interleukin-10 on nitric oxide production in murine macrophages
1996
Opposite effects of interleukin-4 and interleukin-10 on nitric oxide production in murine macrophages
Sample size: 6
publication
Evidence: moderate
Author Information
Author(s): A. Maru, S. K. Jackson
Primary Institution: University of Wales College of Medicine
Hypothesis
How do interleukin-4 and interleukin-10 affect nitric oxide production in activated murine macrophages?
Conclusion
Interleukin-4 inhibits nitric oxide production while interleukin-10 increases it in activated murine macrophages.
Supporting Evidence
- IL-4 pre-treatment significantly inhibited nitric oxide production in macrophages activated by IFN-7 or LPS.
- IL-10 pre-treatment significantly increased nitric oxide production in macrophages activated by IFN-7 or LPS.
- IL-4 did not induce nitric oxide production when used alone.
- IL-10 failed to suppress nitric oxide production in activated macrophages.
Takeaway
This study found that one chemical (IL-4) stops cells from making a substance called nitric oxide, while another chemical (IL-10) makes them produce more of it.
Methodology
Murine macrophage cell lines were treated with IL-4 or IL-10 and then stimulated with IFN-7 or LPS to measure nitric oxide production.
Participant Demographics
Murine macrophage cell lines (RAW 264.7 and J774.2)
Statistical Information
P-Value
p<0.001, p<0.01, p<0.05
Statistical Significance
p<0.001
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