Dasatinib's Effect on Nilotinib-Resistant CML Cells
Author Information
Author(s): Okabe Seiichi, Tauchi Tetsuzo, Tanaka Yuko, Ohyashiki Kazuma
Primary Institution: Tokyo Medical University
Hypothesis
Can dasatinib induce apoptosis in nilotinib-resistant chronic myeloid leukemia cells by inhibiting Lyn kinase?
Conclusion
Dasatinib effectively induces apoptosis in nilotinib-resistant CML cells by inhibiting Lyn kinase activity.
Supporting Evidence
- Dasatinib reduced the cell growth of K562NR and significantly induced apoptosis.
- The IC50 of dasatinib is 5 nM.
- K562NR cells underwent increased phosphorylation of Src family kinases including Lyn.
- Phosphorylation of SFK was reduced after 24-hrs dasatinib treatment in a dose-dependent manner.
Takeaway
This study shows that a medicine called dasatinib can help kill cancer cells that don't respond to another medicine called nilotinib.
Methodology
The study established a nilotinib-resistant cell line and evaluated the resistance to nilotinib and the efficacy of dasatinib.
Limitations
The study was conducted in vitro and may not fully represent in vivo conditions.
Participant Demographics
Patients included in the study had chronic myeloid leukemia and were resistant to imatinib and nilotinib.
Digital Object Identifier (DOI)
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