Comparative Analysis of Secretomes in Trichoderma reesei Strains
Author Information
Author(s): Herpoël-Gimbert Isabelle, Margeot Antoine, Dolla Alain, Jan Gwénaël, Mollé Daniel, Lignon Sabrina, Mathis Hughes, Sigoillot Jean-Claude, Monot Frédéric, Asther Marcel
Primary Institution: INRA, UMR1163, Biotechnologie des Champignons Filamenteux, F-13000 Marseille, France
Hypothesis
How do the secretomes of the hypersecretory strains CL847 and Rut-C30 of Trichoderma reesei differ in composition and enzyme production?
Conclusion
The study reveals significant differences in the secretome composition of two Trichoderma reesei strains, with CL847 showing a more diversified enzyme profile compared to Rut-C30.
Supporting Evidence
- 22 protein species were identified in the secretome of T. reesei.
- CL847 produced a more diverse set of enzymes compared to Rut-C30.
- Significant differences in enzymatic activities were observed between the two strains.
Takeaway
Scientists looked at two types of fungi that help break down plant material. They found that one type makes a wider variety of helpful proteins than the other.
Methodology
The study used two-dimensional electrophoresis and mass spectrometry to analyze the protein composition of the secretomes from the two strains grown under similar conditions.
Limitations
The identification of some proteins was limited due to low biological material and post-translational modifications.
Digital Object Identifier (DOI)
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