Impact of Temperature Increase on E. coli Fermentation and Gene Expression
Author Information
Author(s): Hasan Chowdhurry, Mohammad Monirul, Shimizu Kazuyuki
Primary Institution: Kyushu Institute of Technology
Hypothesis
How does a temperature up-shift from 37°C to 42°C affect the metabolism and gene expression in Escherichia coli?
Conclusion
The study found that increasing the temperature led to metabolic changes in E. coli, including higher acetate production and altered gene expression.
Supporting Evidence
- At 42°C, E. coli produced more acetate and had a lower biomass yield compared to 37°C.
- The glucose uptake rate decreased at higher temperatures due to changes in gene expression.
- The expression of heat shock genes increased significantly during the first 10-20 minutes after the temperature up-shift.
Takeaway
When E. coli is heated from 37°C to 42°C, it makes more acetate and uses sugar less efficiently, which can be important for making proteins.
Methodology
The study involved aerobic batch and continuous cultivations of E. coli at two different temperatures, measuring fermentation parameters and gene expressions.
Limitations
The study did not explore the effects of different dilution rates or heterologous protein production during temperature shifts.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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