How CAR and Sialic Acid Affect Adenovirus Distribution in the Body
Author Information
Author(s): Seiradake Elena, Henaff Daniel, Wodrich Harald, Billet Olivier, Perreau Matthieu, Hippert Claire, Mennechet Franck, Schoehn Guy, Lortat-Jacob Hugues, Dreja Hanna, Ibanes Sandy, Kalatzis Vasiliki, Wang Jennifer P., Finberg Robert W., Cusack Stephen, Kremer Eric J.
Primary Institution: European Molecular Biology Laboratory, Grenoble Outstation, Grenoble, France
Hypothesis
The study investigates how the cell adhesion molecule CAR and sialic acid on human erythrocytes influence the biodistribution of adenoviruses in vivo.
Conclusion
The presence of CAR on erythrocytes leads to prolonged blood half-life and reduced liver infection of CAR-tropic adenoviruses.
Supporting Evidence
- The study provides a molecular and structural rationale for adenovirus-erythrocyte interactions.
- CAR expression on erythrocytes leads to significant differences in adenovirus biodistribution.
- Transgenic mice expressing CAR showed increased viral load in the blood after adenovirus injection.
- Neuraminidase treatment of erythrocytes reduced hemagglutination by adenoviruses.
- Different adenovirus serotypes utilize distinct receptors for erythrocyte binding.
- The study suggests that CAR-tropic adenoviruses may have different infection patterns in various species.
- Binding assays indicated that sialic acid is a key determinant for HAd37 binding to erythrocytes.
- Competition assays demonstrated the role of CAR in CAV-2 agglutination of human erythrocytes.
Takeaway
This study shows that certain viruses can stick to red blood cells, which helps them stay in the body longer and avoid getting into the liver.
Methodology
The study used molecular, biochemical, structural, and transgenic animal-based analyses to characterize adenovirus interactions with erythrocytes.
Potential Biases
Potential bias in the selection of animal models and serotypes used for the study.
Limitations
The study primarily focuses on specific adenovirus serotypes and may not generalize to all adenoviruses.
Participant Demographics
The study involved human erythrocytes and transgenic mice expressing CAR.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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