Expression and Genetic Loss of Function Analysis of the HAT/DESC Cluster Proteases TMPRSS11A and HAT
2011

Study of HAT/DESC Proteases in Mice

Sample size: 161 publication Evidence: moderate

Author Information

Author(s): Katiuchia Uzzun Sales, John P. Hobson, Rebecca Wagenaar-Miller, Roman Szabo, Amber L. Rasmussen, Alexandra Bey, Maham F. Shah, Alfredo A. Molinolo, Thomas H. Bugge

Primary Institution: National Institute of Dental and Craniofacial Research, National Institutes of Health

Hypothesis

What are the physiological functions of the HAT/DESC subfamily of proteases?

Conclusion

The study found that TMPRSS11A and HAT are not essential for development, health, or long-term survival in mice.

Supporting Evidence

  • Transcripts of HAT/DESC proteases were found in many organs of both mice and humans.
  • Mutant mice lacking TMPRSS11A and HAT showed no significant health issues.
  • Prostate hyperplasia was observed in some TMPRSS11A-deficient mice.
  • Lymphoma incidence was lower in TMPRSS11d+/+ females compared to TMPRSS11dāˆ’/āˆ’ females.

Takeaway

Scientists studied certain proteins in mice to see if they were needed for growth and health, and found that the mice did just fine without them.

Methodology

The study involved gene targeting in mice to create mutant strains lacking TMPRSS11A and HAT, followed by phenotypic analysis.

Limitations

The study primarily focused on two specific proteases and may not represent the full functional redundancy of the entire HAT/DESC subfamily.

Participant Demographics

Mice used in the study were of mixed genotypes, including both male and female subjects.

Statistical Information

P-Value

p>0.05

Statistical Significance

p>0.05

Digital Object Identifier (DOI)

10.1371/journal.pone.0023261

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