Composition and Acidification of the Culture Medium Influences Chronological Aging Similarly in Vineyard and Laboratory Yeast
2011

How Yeast Aging Works in the Lab and Vineyard

publication Evidence: moderate

Author Information

Author(s): Christopher J. Murakami, Valerie Wall, Nathan Basisty, Matt Kaeberlein

Primary Institution: University of Washington

Hypothesis

Are the mechanisms of chronological aging similar in vineyard and laboratory yeast strains?

Conclusion

The study found that vineyard yeast RM11 and laboratory yeast BY4743 exhibit similar chronological aging properties.

Supporting Evidence

  • Both RM11 and BY4743 showed increased chronological life span from dietary restriction.
  • Buffering the culture medium to pH 6 significantly extended the chronological life span of both strains.
  • The study found that acidification of the culture medium limits chronological life span in both vineyard and laboratory strains.

Takeaway

This study shows that both vineyard and lab yeast age in similar ways, which means what we learn from lab yeast can help us understand aging in nature too.

Methodology

The study compared chronological aging in vineyard yeast RM11 and laboratory yeast BY4743 under various conditions, measuring survival and pH changes.

Potential Biases

Potential bias due to the limited genetic diversity of the yeast strains studied.

Limitations

The study primarily focused on two yeast strains and may not represent all yeast species.

Participant Demographics

The study involved two yeast strains: RM11 from a vineyard and BY4743 from a laboratory.

Statistical Information

P-Value

2.0×10−5

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pone.0024530

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