Chromosomal Aberrations in Bladder Cancer: Fresh versus Formalin Fixed Paraffin Embedded Tissue and Targeted FISH versus Wide Microarray-Based CGH Analysis
Author Information
Author(s): Panzeri Elena, Conconi Donatella, Antolini Laura, Redaelli Serena, Valsecchi Maria Grazia, Bovo Giorgio, Pallotti Francesco, Viganò Paolo, Strada Guido, Dalprà Leda, Bentivegna Angela
Primary Institution: Department of Neuroscience and Biomedical Technologies, University of Milan-Bicocca, Monza, Italy
Hypothesis
The study aims to evaluate the performance of the UroVysion assay for detecting Transitional Cell Carcinoma (TCC) in different types of tissue samples.
Conclusion
The study found that UroVysion test results were generally comparable between freshly isolated nuclei and formalin-fixed paraffin-embedded tissues, but some differences were noted in high-grade tumors.
Supporting Evidence
- The UroVysion assay showed comparable results between fresh and fixed tissue samples for low-grade tumors.
- Significant differences were observed in high-grade tumors for some chromosomal probes.
- Array-CGH identified numerous genetic changes, including frequent losses on chromosome 9p.
Takeaway
Researchers looked at bladder cancer samples to see if a specific test worked well on fresh and preserved tissues, and they found it worked similarly in most cases.
Methodology
The study compared UroVysion test results on freshly isolated nuclei and formalin-fixed paraffin-embedded tissues from bladder cancer samples, and also assessed concordance with array-CGH profiles.
Limitations
The study noted limitations in detecting low-level mosaicism and changes in copy number due to cellular heterogeneity.
Participant Demographics
28 men and 4 women with newly diagnosed TCCs.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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