Using BOX-PCR to Identify Melioidosis Outbreaks
Author Information
Author(s): Currie Bart J, Gal Daniel, Mayo Mark, Ward Linda, Godoy Daniel, Spratt Brian G, LiPuma John J
Primary Institution: Northern Territory Clinical School, Flinders University, Royal Darwin Hospital, Darwin, Northern Territory, Australia
Hypothesis
Can BOX-PCR effectively distinguish between clonal and non-clonal outbreaks of melioidosis?
Conclusion
BOX-PCR can be used to exclude a clonal outbreak of melioidosis within 10 hours of receiving the bacterial strains.
Supporting Evidence
- BOX-PCR typing compared favourably with MLST and PFGE.
- BOX-PCR can usually discriminate between non-clonal isolates.
- BOX-PCR can show relatedness within clonal groups.
Takeaway
Scientists found a quick way to tell if a group of melioidosis cases comes from the same source, which helps in stopping outbreaks faster.
Methodology
The study adapted BOX-PCR for typing B. pseudomallei and compared its results with MLST and PFGE on the same isolates.
Limitations
BOX-PCR may be less reproducible than PFGE and results can vary between different PCR machines.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website