Gene Expression Changes in Dictyostelium During Phagocytosis
Author Information
Author(s): Sillo Alessio, Bloomfield Gareth, Balest Alessandra, Balbo Alessandra, Pergolizzi Barbara, Peracino Barbara, Skelton Jason, Ivens Alasdair, Bozzaro Salvatore
Primary Institution: Department of Clinical and Biological Sciences, University of Turin
Hypothesis
How does phagocytosis affect gene expression in Dictyostelium?
Conclusion
Phagocytosis induces significant changes in gene expression related to metabolism and cellular processes in Dictyostelium.
Supporting Evidence
- 443 genes are differentially regulated by phagocytosis.
- Phagocytosis induces changes in carbohydrate, amino acid, and lipid metabolism.
- Very few changes were detected in genes required for vesicle fission/fusion.
Takeaway
When Dictyostelium cells eat bacteria, they change how they use their genes to help them grow and fight off germs.
Methodology
DNA microarrays were used to analyze gene expression in Dictyostelium cells during phagocytosis and growth on bacteria.
Limitations
The study may not account for all variables affecting gene expression during phagocytosis.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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