Using Laccase Enzymes to Detect Methylene Blue in Water
Author Information
Author(s): Sánchez-Álvarez Araceli, Quintanilla-Villanueva Gabriela Elizabeth, Rodríguez-Quiroz Osvaldo, Rodríguez-Delgado Melissa Marlene, Villarreal-Chiu Juan Francisco, Sicardi-Segade Analía, Luna-Moreno Donato
Primary Institution: Electromecánica Industrial, Universidad Tecnológica de León
Hypothesis
Can laccase enzymes be effectively used as bio-receptors for detecting methylene blue in a biosensor?
Conclusion
The study successfully developed a biosensor that detects methylene blue with a limit of detection of 4.61 mg L−1 and a recovery rate of 122.46 ± 4.41% from spiked rainwater samples.
Supporting Evidence
- The biosensor maintained a stable signal over 17 cycles and was effective for 30 days at room temperature.
- The method demonstrated a limit of detection of 4.61 mg L−1 and a limit of quantification of 15.37 mg L−1.
- A spiked rainwater sample yielded a recovery rate of 122.46 ± 4.41%.
Takeaway
Scientists created a special sensor that uses enzymes to find a harmful dye in water, showing it works well and can be reused.
Methodology
The study involved developing a Surface Plasmon Resonance biosensor using laccases immobilized on a gold surface to detect methylene blue.
Limitations
The limit of detection is higher than some conventional methods, and the study suggests that further optimization could improve sensitivity.
Statistical Information
P-Value
4.61 mg L−1
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website