Purification and characterisation of a breast-cancer-associated glycoprotein not expressed in normal breast and identified by monoclonal antibody 83D4
1991
Breast Cancer Glycoprotein Identified by Monoclonal Antibody 83D4
publication
Evidence: moderate
Author Information
Author(s): G. Pancino, E. Osinagal, C. Charpin, D. Mistrol, J.Ph. Barquel, A. Roseto
Primary Institution: Division d'Immunocytologie Appliquee, Universite de Compiegne
Hypothesis
The study aims to purify and characterize the antigen identified by monoclonal antibody 83D4.
Conclusion
The antigen identified by mAb 83D4 is a unique glycoprotein associated with breast cancer that is not present in normal breast tissue.
Supporting Evidence
- Monoclonal antibody 83D4 was generated using human breast carcinoma tissue.
- 83D4 was reactive with breast carcinoma tissues but not with normal breast.
- The antigen was detected in various breast cancer cell lines and tissues.
- Purified antigen showed high molecular weight and glycoprotein characteristics.
- 83D4 antigen did not react with normal human milk components.
Takeaway
Researchers found a special protein linked to breast cancer that normal breast tissue doesn't have, using a specific antibody.
Methodology
The antigen was purified from various sources including breast cancer cell lines and pleural effusion fluids, and analyzed using biochemical and immunological methods.
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