SNP Transferability in Grapevines
Author Information
Author(s): Silvia Vezzulli, Diego Micheletti, Summaira Riaz, Massimo Pindo, Roberto Viola, Patrice This, Andrew M Walker, Michela Troggio, Riccardo Velasco
Primary Institution: IASMA Research Centre
Hypothesis
Can SNP markers from the heterozygous grape cultivar Pinot Noir be effectively transferred to other grape varieties and species?
Conclusion
SNP markers from the Pinot Noir cultivar are informative for V. vinifera but have limited application for non-vinifera Vitis species.
Supporting Evidence
- The study genotyped 137 SNPs across 69 grape accessions.
- Transferability of SNPs was found to be 31.5% among unrelated V. vinifera cultivars.
- Only 2.3% transferability was observed among non-vinifera Vitis species.
Takeaway
Scientists studied grape DNA to see if certain genetic markers could be used in different grape types. They found that some markers work well in related grapes but not in others.
Methodology
The study involved genotyping 137 SNPs using the SNPlex™ Genotyping System and sequencing 35 regions across 69 grape accessions.
Limitations
The transferability of SNPs was lower in wild forms and non-vinifera species, suggesting a need for resequencing strategies.
Participant Demographics
The study included 71 grape accessions, comprising 24 V. vinifera cultivars, 10 wild forms, and 37 non-vinifera accessions.
Digital Object Identifier (DOI)
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