Proteases from Acanthamoeba and Their Role in Brain Invasion
Author Information
Author(s): Sissons James, Alsam Selwa, Goldsworthy Graham, Lightfoot Mary, Jarroll Edward L, Khan Naveed Ahmed
Primary Institution: School of Biological and Chemical Sciences, Birkbeck College, University of London, England, UK
Hypothesis
Could Acanthamoeba proteases be involved in the invasion of the central nervous system?
Conclusion
Acanthamoeba proteases degrade extracellular matrix, which may facilitate the invasion of brain tissue by the amoebae.
Supporting Evidence
- The study identified two major proteases from an Acanthamoeba isolate.
- Both proteases were shown to degrade components of the extracellular matrix.
- The serine protease caused disruptions in human brain microvascular endothelial cell monolayers.
Takeaway
Acanthamoeba has special proteins that can break down parts of the brain, which might help it get inside and cause sickness.
Methodology
The study used zymographic assays to analyze protease activity and cytotoxicity assays to assess effects on human brain microvascular endothelial cells.
Limitations
Only two proteases were observed, and other proteases may exist that are sensitive to the experimental conditions used.
Digital Object Identifier (DOI)
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