Carbenoxolone Blocks Calcium Rise in Retinal Ganglion Cells
Author Information
Author(s): Bramley Jayne R., Wiles Erin M., Sollars Patricia J., Pickard Gary E.
Primary Institution: School of Veterinary Medicine and Biomedical Sciences, University of Nebraska, Lincoln, Nebraska, United States of America
Hypothesis
Does carbenoxolone inhibit light-evoked calcium responses in melanopsin-expressing retinal ganglion cells?
Conclusion
Carbenoxolone inhibits the light-evoked rise in intracellular calcium in isolated melanopsin ganglion cell photoreceptors.
Supporting Evidence
- Carbenoxolone completely abolished the light-evoked rise in intracellular calcium in isolated ipRGCs.
- Recovery from carbenoxolone inhibition was variable, with some cells showing no recovery.
- Carbenoxolone did not inhibit light-evoked action potential firing in ipRGCs in situ.
Takeaway
Carbenoxolone is a drug that stops certain cells in the eye from responding to light by blocking calcium signals, which are important for their function.
Methodology
The study used fura-2 imaging to examine light-evoked calcium responses in isolated rat ipRGCs in the presence and absence of carbenoxolone.
Limitations
Recovery from carbenoxolone inhibition was variable among cells.
Participant Demographics
Isolated melanopsin-expressing retinal ganglion cells from neonatal rats.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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