Role of RGM coreceptors in bone morphogenetic protein signaling
2007
Role of RGM co-receptors in BMP signaling
Sample size: 4
publication
Evidence: high
Author Information
Author(s): Halbrooks Peter J, Ding Ru, Wozney John M, Bain Gerard
Primary Institution: Genzyme Corporation and Wyeth Discovery Research
Hypothesis
Do RGMa, RGMb, and RGMc play required roles in BMP and TGFβ signaling?
Conclusion
The RGMs are necessary for significant BMP signaling but do not affect TGFβ signaling.
Supporting Evidence
- Knockdown of RGMa or RGMc significantly inhibits BMP2 and BMP12 signaling.
- RGM co-receptors bind tightly to BMP2 and BMP12 with low nanomolar affinity.
- Transient overexpression of RGMa or RGMc increases BMP signaling.
Takeaway
The study found that certain proteins help signals for bone growth work better, but they don't help with other signals.
Methodology
The study used siRNA to knock down RGM proteins in C2C12 cells and assessed BMP and TGFβ signaling through luciferase and alkaline phosphatase assays.
Participant Demographics
C2C12 mouse myoblast cell line
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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