Understanding Protein Function and Sequence Relationships
Author Information
Author(s): Sangar Vineet, Blankenberg Daniel J, Altman Naomi, Lesk Arthur M
Primary Institution: The Pennsylvania State University
Hypothesis
The study investigates the relationship between sequence divergence and function divergence in homologous proteins.
Conclusion
The study reveals that for proteins with more than 50% sequence similarity, the chance of incorrect functional annotation is low.
Supporting Evidence
- The study analyzed 6828 PFAM families to assess sequence and function divergence.
- Results indicate that above 50% sequence identity, erroneous functional annotation occurs in fewer than 6% of cases.
- The research provides a quantitative framework for understanding protein function evolution.
Takeaway
This study looks at how similar proteins can have different functions and helps us understand when we can trust transferring information about protein functions.
Methodology
The study analyzed sequence and functional divergence in 6828 protein families using pairwise alignments and Gene Ontology classifications.
Potential Biases
There is a risk of incorrect annotations due to reliance on sequence similarity alone.
Limitations
The study may not account for all exceptions in functional divergence due to recruitment and other factors.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website