Evaluating MLVA Typing for Bovine Mastitis Pathogen
Author Information
Author(s): Gilbert Florence B, Fromageau Angélina, Lamoureux Jérémy, Poutrel Bernard
Primary Institution: INRA, UR1282, Infectiologie Animale et Santé Publique IASP, F-37380 Nouzilly, France
Hypothesis
Can MLVA typing effectively differentiate Streptococcus uberis strains isolated from bovine mastitis?
Conclusion
The MLVA method can be applied to S. uberis genotyping and is a useful complement to existing typing methods.
Supporting Evidence
- Twenty-five tandem repeats were identified and amplified from 24 S. uberis strains.
- A total of 82 MLVA types were obtained from 88 isolates.
- MLVA typing distinguished strains from different herds and areas.
- The method is low cost and easy to perform.
Takeaway
Scientists found a new way to tell different types of a germ that makes cows sick, which can help farmers keep their cows healthy.
Methodology
The study analyzed the genomic sequence of S. uberis for tandem repeats and used PCR to amplify and type 88 isolates.
Limitations
The study may not cover all genetic variability of S. uberis strains.
Participant Demographics
Isolates were collected from 61 mastitic dairy cows in 59 herds across France.
Digital Object Identifier (DOI)
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