Inhibition of HIV Env Binding by Monoclonal Antibody 2G12
Author Information
Author(s): James M Binley, Stacie Ngo-Abdalla, Penny Moore, Michael Bobardt, Udayan Chatterji, Philippe Gallay, Dennis R Burton, Ian A Wilson, John H Elder, Aymeric de Parseval
Primary Institution: Torrey Pines Institute for Molecular Studies
Hypothesis
Can monoclonal antibody 2G12 inhibit the binding of HIV envelope to cellular receptors?
Conclusion
The study found that 2G12 effectively inhibits HIV Env binding to CCR5 and DC-SIGN, suggesting its potential use in preventing HIV dissemination.
Supporting Evidence
- 2G12 inhibited Env-CCR5 binding and partially inhibited Env-DC-SIGN binding.
- 2G12 showed no effect on Env-syndecan association.
- 2G12 inhibited Env attachment to primary monocyte-derived dendritic cells.
Takeaway
Scientists tested an antibody called 2G12 to see if it could stop HIV from attaching to cells. They found that it works well at blocking this attachment.
Methodology
The study used binding assays with Fc-fusion proteins of gp120 to assess the inhibitory effects of various monoclonal antibodies on HIV Env binding to different cellular receptors.
Limitations
The study may not fully represent the complex interactions in vivo due to the use of simplified binding assays.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website