Inhibition of Tissue Factor Expression in Brain Cells by Nanoparticles
Author Information
Author(s): Wang Huafang, Hu Yu, Guo Tao, Mei Heng, Zhang Xiaoping, Sun Wangqiang
Primary Institution: Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science & Technology
Hypothesis
Can NF-κB decoy oligonucleotides delivered by nanoparticles inhibit tissue factor expression in brain microvascular endothelial cells?
Conclusion
The study found that NF-κB decoy oligonucleotides delivered by PLA nanoparticles effectively reduced tissue factor expression in brain microvascular endothelial cells.
Supporting Evidence
- PLA nanoparticles were shown to be non-toxic to brain microvascular endothelial cells.
- The encapsulation efficacy of decoy oligonucleotides in nanoparticles was 60.5%.
- Cellular uptake of nanoparticles was dependent on incubation time and concentration.
- Decoy oligonucleotides retained their biological activity after being released from nanoparticles.
Takeaway
Researchers used tiny particles to deliver special molecules that can stop a harmful protein in brain cells, which could help treat strokes.
Methodology
The study involved culturing rat brain microvascular endothelial cells and treating them with NF-κB decoy oligonucleotides encapsulated in PLA nanoparticles, followed by various assays to measure tissue factor expression.
Limitations
The study was conducted in vitro, and further research is needed to evaluate the effectiveness in vivo.
Participant Demographics
Rat brain microvascular endothelial cells were used in the study.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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