TEAD Family Transcription Factors Regulate Myf5 Expression in Mouse Embryos
Author Information
Author(s): Ribas Ricardo, Moncaut Natalia, Siligan Christine, Taylor Kevin, Cross Joe W., Rigby Peter W.J., Carvajal Jaime J.
Primary Institution: The Institute of Cancer Research
Hypothesis
A TEAD binding site is required for the activation of Myf5 expression in specific regions of the mouse embryo.
Conclusion
The ECR111 enhancer is essential for Myf5 expression in ventral somitic compartments, and its activity depends on a TEAD binding site.
Supporting Evidence
- ECR111 drives Myf5 expression in specific regions of the mouse embryo.
- A TEAD binding site is essential for the activity of the ECR111 enhancer.
- TEAD proteins localize to the myotome and dermomyotome during early development.
- Deletion of ECR111 abolishes Myf5 expression in certain somitic regions.
Takeaway
Scientists found a special part of DNA that helps control a gene important for muscle development in baby mice, and it needs a specific protein to work.
Methodology
Transgenic analysis, EMSA, and ChIP assays were used to study the enhancer's function and the binding of TEAD proteins.
Limitations
The study does not specify the exact TEAD family members binding to the enhancer due to antibody specificity issues.
Digital Object Identifier (DOI)
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