MicroRNA Presence in Human Mitochondria
Author Information
Author(s): Barrey Eric, Saint-Auret Gaelle, Bonnamy Blandine, Damas Dominique, Boyer Orane, Gidrol Xavier
Primary Institution: Unité de Biologie Intégrative des Adaptations à l'Exercice – INSERM U902, Genopole Evry, France
Hypothesis
Some miRNA could be present in the mitochondria for post-transcriptomic regulation by RNA interference.
Conclusion
The study demonstrated the presence of pre-miRNA and miRNA in human mitochondria isolated from skeletal muscular cells.
Supporting Evidence
- Twenty five human pre-miRNA and 33 miRNA alignments were found in the reference mitochondrial sequence.
- In situ hybridization demonstrated that these miRNA were localized in mitochondria of human myoblasts.
- Forty six miRNA were significantly expressed for the smallest RNA input concentration.
Takeaway
Scientists found tiny molecules called microRNAs in the powerhouses of our cells, which help control how genes work.
Methodology
In-silico analysis and in situ hybridization were used to identify and confirm the presence of miRNA in mitochondria.
Limitations
The origin of the detected miRNA remains unclear and requires further investigation.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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