Effects of Acidic pH Shock on Stress-Response Genes in Streptomyces coelicolor
Author Information
Author(s): Kim Yoon Jung, Moon Myung Hee, Song Jae Yang, Smith Colin P, Hong Soon-Kwang, Chang Yong Keun
Primary Institution: Korea Advanced Institute of Science and Technology
Hypothesis
How does acidic pH shock affect the expression of sigma factors and shock-related proteins in S. coelicolor A3(2)?
Conclusion
Acidic pH shock significantly upregulates a variety of sigma factors and stress-related proteins, enhancing actinorhodin production.
Supporting Evidence
- The acidic pH shock led to the upregulation of sigma factors like sigH, sigR, and hrdD.
- Heat shock proteins and oxidative stress-related proteins were also significantly upregulated.
- The study demonstrated that actinorhodin production increased significantly in pH-shocked cultures.
Takeaway
When the bacteria experience a sudden drop in pH, they react by turning on many genes that help them cope with stress, which helps them make more of a special substance called actinorhodin.
Methodology
Transcriptional and proteomic analyses were performed to assess gene expression changes in response to acidic pH shock.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website