EAAC1: The High-Affinity L-Aspartate Transporter in Prostate Cells
Author Information
Author(s): Franklin Renty B, Zou Jing, Yu Ziqiang, Costello Les C
Primary Institution: Department of Biomedical Sciences/Dental School, University of Maryland, Baltimore, Maryland, USA
Hypothesis
Is EAAC1 the functional high-affinity L-aspartate transporter in prostate cells?
Conclusion
EAAC1 functions as the high-affinity L-aspartate transporter in prostate cells and is regulated by testosterone and prolactin.
Supporting Evidence
- EAAC1 is expressed in both normal and hyperplastic human prostate glands.
- Testosterone and prolactin significantly increase the high-affinity transport of aspartate.
- EAAC1 expression is down-regulated by EAAC1 siRNA knock down.
Takeaway
Prostate cells need a special transporter to take in aspartate, which helps them make citrate. This transporter, called EAAC1, works better when testosterone and prolactin are around.
Methodology
The study involved RT-PCR to assess EAAT expression in prostate cells and human prostate cell lines, along with aspartate uptake assays and siRNA knock-down experiments.
Limitations
The study primarily focuses on EAAC1 without exploring other potential transport mechanisms in prostate cells.
Participant Demographics
The study involved rat and human prostate epithelial cells.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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