Tyrosine Phosphorylation of Ugd in E. coli and Its Role in Colanic Acid Synthesis and Polymyxin Resistance
Author Information
Author(s): Lacour Soline, Bechet Emmanuelle, Cozzone Alain J., Mijakovic Ivan, Grangeasse Christophe
Primary Institution: Institut de Biologie et Chimie des Protéines, University of Lyon, CNRS, Lyon, France
Hypothesis
Does Ugd phosphorylation by Wzc and Etk affect colanic acid production and polymyxin resistance in E. coli?
Conclusion
Ugd phosphorylation is crucial for regulating both colanic acid synthesis and polymyxin resistance in E. coli.
Supporting Evidence
- Ugd is phosphorylated by both Wzc and Etk, indicating a regulatory mechanism.
- Phosphorylation of Ugd on Tyr71 affects colanic acid production.
- Ugd phosphorylation by Etk is linked to resistance against polymyxin.
- Mutations in Ugd that prevent phosphorylation lead to reduced colanic acid synthesis.
- Ugd's role in producing UDP-glucuronic acid is essential for polysaccharide biosynthesis.
Takeaway
This study shows that a protein in E. coli can be modified in a way that helps the bacteria make a protective coating and resist certain antibiotics.
Methodology
The study involved confirming Ugd phosphorylation by Wzc and Etk in vivo and assessing the effects of this phosphorylation on colanic acid production and polymyxin resistance.
Digital Object Identifier (DOI)
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