Resurrecting DNA Function from an Extinct Species
Author Information
Author(s): Pask Andrew J., Behringer Richard R., Renfree Marilyn B.
Primary Institution: University of Texas M. D. Anderson Cancer Center
Hypothesis
Can we restore and examine the function of non-coding DNA from extinct species in vivo?
Conclusion
The study successfully demonstrated that a non-coding DNA fragment from the extinct thylacine can drive gene expression in mice, indicating its conserved role in cartilage formation.
Supporting Evidence
- The thylacine enhancer directed chondrocyte-specific expression in transgenic mice.
- This is the first example of restoring extinct non-coding DNA function in vivo.
- The study provides insights into gene evolution and diversity.
Takeaway
Scientists took DNA from a long-gone animal called the thylacine and showed that it can still work in mice, helping us understand how genes from extinct animals might have functioned.
Methodology
The researchers isolated a transcriptional enhancer from thylacine DNA and used a transgenic approach to test its function in mice.
Limitations
The variations in transgene expression levels may affect the interpretation of results, and the precise function of the enhancer in its native context remains uncertain.
Digital Object Identifier (DOI)
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