Culture and Maintenance of Human Embryonic Stem Cells
2009
How to Care for Human Embryonic Stem Cells
publication
Author Information
Author(s): Kent Lia
Primary Institution: Stemgent
Conclusion
Proper maintenance and cleaning of human embryonic stem cell cultures are essential for keeping them healthy and undifferentiated.
Supporting Evidence
- Human embryonic stem cells need daily feeding to maintain their health.
- Differentiated areas in stem cell cultures must be manually removed to keep the cultures undifferentiated.
- Cultures should be passaged every 4-7 days to prevent overcrowding and excessive differentiation.
Takeaway
To keep human embryonic stem cells healthy, you need to feed them every day and remove any parts that start to change into other types of cells.
Methodology
The study outlines protocols for observing, feeding, and cleaning human embryonic stem cell cultures.
Digital Object Identifier (DOI)
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